Structural studies on the interaction of nano-SiO2 with lysozyme

Authors

  • B. Shareghi Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
  • M. Salavati-Niasari Institute of Nano Science and Nano Technology, University of Kashan,Kashan,P.O.BOX.87317-51167,I. R. of Iran
  • S. Farhadian Department of Biology, University of Shahrekord, P.O. Box: 115, Shahrekord, I. R. of Iran
Abstract:

The interaction between nano-SiO2 and lysozyme was investigated by the method of UV-Visible  detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-SiO2 over the temperature range (293-373) K in different buffers and pH values, using temperature scanning spectroscopy. The presence of nano-SiO2 caused the destabilization of lysozyme resulting in a decrease in the temperature of unfolding with an increase in nano-SiO2 concentration.

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Journal title

volume 1  issue 3

pages  205- 212

publication date 2011-07-01

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